METABOLIC ENGINEERING

METABOLIC ENGINEERING Question Papers (Supple, 2006)
SET : 1

1. What is bio-conversion? Discuss the advantages of bioconversion.

2. What is permeability? Discuss the alteration of permeability.

3. Discuss the biosynthesis of secondary metabolites.

4. What is growth cycle? How do you recognize growth cycle peaks?

5. Explain bio-conversion of insoluble substances.

Write about cAMP deficiency

6. Write short notes on the following:

a) Jacob Monod model

b) Passive diffusion

c) Resistant mutants

d) Catbolic repression

7. Describe the amino acid regulation of RNA synthesis.

8. Write short notes on :

a) Mutation

b) Cometabolism

c) Gene dosage

d) Feed back regulation


SET : 2

1. Define bio-conversion and what are the factors important to bio-conversions?

2. What is catabolic repression? Discuss mutants resistant to repression.

3. Describe the catabolite regulation in secondary metabolism?

4. Discuss the synthesis of primary metabolites.

5. Wtite about :

a) Differential regulation by isozymes.

b) Explain feed back regulation.

6. Write short notes on the following :

a) Precursor effect

b) Gene dosage

c) Co-metabolism

d) Permeability

7. Describe the metabolic regulation in branched pathways.

8. Write short notes on the following :

a) Resistant mutants

b) Diffusion

c) Mutation

d) Enzyme induction


SET : 3

1. Discuss the regulation of enzyme synthesis.

2. What is fermentation? What steps do you suggest for improving fermentation.

3. Discuss the biosynthesis of secondary metabolites.

4. What is permeability? Discuss the alteration of permeability.

5. Write about the following :

a) Feed back repression

b) Bioconversion of insoluble substances

6. Write short notes on the following :

a) Jacob Monod model

b) Bio-conversion

c) Gene dosage

d) Precursor effect

7. Describe the amino acid regulation of RNA synthesis.

8. Write short notes on the following :

a) Co-metabolism

b) Mutation

c) Diffusion

d) Strain selection.


SET : 4

1. Discuss the avoidance of product inhibition.

2. Discuss the role of strain in improving fermentation with some examples.

3. Explain the procedures of secondary metabolites.

4. What is permeability? Discuss the alteration of permeability.

5. Write about differential regulation by isozymes.

Define diffusion? Explain passive diffusion and facilitated diffusion.

6. Write short notes on the :

a) Jacob Monod model

b) Precursor effect

c) Catabolic repression

d) Gene dosage

7. Describe amino acid regulation of RNA synthesis.

8. Write short notes on the following :

a) Feed back regulation

b) Mutation

c) Bio-conversions

d) cAMP deficiency

POSTED BY VAGDEVI AT 11:59 PM 0 COMMENTS
LABELS: METABOLIC ENGIEERING
REACTIONS:
METABOLIC ENGINEERING Question Papers (Regular, 2007)
SET : 1

1. Describe in detail positive and negative control of gene expression by citing the example of lac operon.

2. Write about different enzymes required for the synthesis of cAMP.

3. How do you apply the principle of metabolic engineering to the synthesis of tryptophan. Explain in detail.

4. Discuss the different parameters required for limiting end product accumulation.

5. Write notes on :

a) Secondary metabolites

b) Idiophase.

6. How does carry out the bioconversion reaction for insoluble substrate. Discuss in detail.

7. Discuss different kinds of bioconversions with suitable examples.

8. Explain the phenomena of feed back repression in resistant mutants.

POSTED BY VAGDEVI AT 11:57 PM 0 COMMENTS
LABELS: METABOLIC ENGIEERING
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FRIDAY, OCTOBER 17, 2008

METABOLIC ENGINEERING supply 2007)
SET :1
1. Explain the Jacob Monod model of induction and also regulation of the operon by CAMP. [16]
2. What do you understand by feed back regulation? Explain this with special reference to amino acid biosynthetic pathways. [16]
3. How can you overcome feed back regulation? Explain some strategies by which one can maximize the production of a metabolite of interest. [16]
4. Define primary and secondary metabolites. What is the difference between the two? Give examples for both of them. [16]
5. What are bioconversions? How are these important in synthesis? Explain with specific examples. [16]
6. Explain two of the following:(a) Cometabolism(b) Precursor effects in metabolite synthesis [16]
7. Discuss the different strain improvement methods that are currently being used. [16]
8. Write notes on:(a) Repression(b) Induction(c) Regulation of enzyme synthesis(d) Alteration in permeability. [16]
SET :2
1. Explain the operon concept using Lac and Trp operons as examples. [16]
2. What do you understand by membrane transport? Explain the different transport processes in detail. [16]
3. What is Feed back regulation? How can one overcome feedback regulation? [16]
4. Explain any two of the following:(a) Catabolite repression(b) Alteration in cell permeability(c) Cometabolism. [16]
5. Explain in detail the different strategies that can be adopted for maximizing the yields of secondary metabolite. [16]
6. Distinguish between primary and secondary metabolites. Give at least four examples each for each of them. 1[16]
7. What are the different methods used for strain improvement? [16]
8. Write notes on:(a) Gene dosage(b) Mixed or sequential bioconversions(c) Advantages of bioconversions(d) Isozymes. [16]
SET :3
1. Explain in detail the regulation of amino acid biosynthetic operons. [16]
2. Explain the following:(a) Passive diffusion(b) Active transport(c) Feed back regulation. [16]
3. What are primary and secondary metabolites. Distinguish between the two and give at least four examples for each type of metabolite. [16]
4. What are Bioconversions? What are the factors that effect bioconversions. Discuss its advantages over chemical synthesis. [16]
5. Write about the following in detail:(a) Mutations(b) Gene dosage. [16]
6. Comment on:(a) Importance of altering membrane permeability.(b) Isoenzymes and their role in regulation. [16]
7. What are the different strategies used for strain improvement? Discuss in detail [16]
8. Write short notes on:(a) Mutants resitant to repression(b) Induction(c) Catabolite repression.(d) Conversion of insoluble substances. [16]

SET :4
1. Explain in detail the Jacob Monod model of gene regulation by using the example of Lac and Trp operons. [16]
2. Explain the different modes of membrane transport. [16]
3. What are primary metabolites? How do you formulate different strategies for enhancing the production of primary metabolites.
4. What are secondary metabolites? Explain the phase during which it is synthesized and the methods. [16]
5. Discuss the factors influencing bioconversions. [16]
6. How can one improve the yields of a desired product by both genetic and fermentation methods. [16]
7. Write short notes on:(a) Bioconversion of insoluble products(b) Feed back inhibition. [16]
8. Comment on:(a) Catabolite repression(b) Gene dosage. [16]
POSTED BY VAGDEVI AT 6:01 AM 0 COMMENTS
LABELS: METABOLIC ENGIEERING
REACTIONS:
METABOLIC ENGINEERING-REG 2K5
Code No: RR412311 Set No.1
IV B.Tech. I Semester Regular Examinations, November -2005
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write short notes on:
(a) Passive diffusion
(b) Facilitated diffusion [16]
2. Describe in detail the primary screening involved in strain selection with example.
[16]
3. Describe
(a) Differential regulation by ISO enzymes.
(b) Gene dosage. [16]
4. Explain mixed or sequential Bioconversions with suitable examples. [16]
5. Describe Induction / Repression phenomena in E.coli with examples. [16]
6. Describe Enzyme inhibition and factors involved in it. [16]
7. How does mutation effect the enzyme production? List out the factors involved in
optimization of mutants for high yield protein production? [16]
8. Explain the Fermentation parameters involved in production of wine from yeast.
[16]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.2
IV B.Tech. I Semester Regular Examinations, November -2005
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write short notes on:
(a) Feed back repression
(b) CAMP [16]
2. Describe in detail the secondary screening involved in strain selection with example.
[16]
3. Detail the regulation of Enzyme synthesis at Fermentor level. [16]
4. Define Bioconversion and describe in detail the conversion of insoluble substances
by sequential bioconversion. [16]
5. Describe induction and Repression phenomena in yeast, citing Alcohol production
as example. [16]
6. Describe in detail the various modes of diffusion? [16]
7. What is enzyme inhibition and detail the various modes of enzyme inhibition.[16]
8. Distinguish and differentiate concerted feed back regulation and cumulative feed
back regulation with examples. [16]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.3
IV B.Tech. I Semester Regular Examinations, November -2005
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write short notes on:
(a) Cometabolism during Bioconversion
(b) Feed back regulation. [16]
2. List out and describe the parameters involved in scale up of Fermentation (large
scale) from pilot scale. [16]
3. What is bio conversion and what are the advantages of molecules generated by bio
conversion to industry. [16]
4. Explain gene regulation by Jacob and Monad model citing lac operon as example.
[16]
5. Evaluate catabolite regulation with tryptophan operon as example. [16]
6. Explain how gene dosage is evaluated and how does gene dosage effect Fermentation
process. [16]
7. List out the biotechnological application of enzymes (eg:- Proteases, Amylases etc)
Produced by Fermentation. [16]
8. Distinguish and differentiate concerted and cumulative feed back regulation with
examples. [16]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.4
IV B.Tech. I Semester Regular Examinations, November -2005
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write short notes on:
(a) Strain selection
(b) Isozymes. [16]
2. How can Metabolic pathways be genetically controlled with examples. [16]
3. What is Bioconversion and describe the factors involved in Bioconversion? [16]
4. Describe:
(a) Concerted feed back regulation
(b) Amino acid regulation. [16]
5. Describe the factors contributing to catalytic efficiency of an enzyme. [16]
6. Define Mutation and various modes of generating mutations in improving industrial
biotechnology of an organism? [16]
7. Compare and contrast direct and indirect fermentations citing amino acid synthesis
as example? [16]
8. Describe
(a) Precursor effects in biosynthesis of secondary metabolites.
(b) Producers of secondary metabolites. [16]
? ? ? ? ?
1 of 1
POSTED BY VAGDEVI AT 5:57 AM 0 COMMENTS
LABELS: METABOLIC ENGIEERING
REACTIONS:
METABOLIC ENGINEERING-REG 2K6
Code No: RR412311 Set No.1
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Describe in detail the primary screening involved in strain selection with an exam-
ple. [16]
2. Write short notes on:
(a) Co-metabolism during bio-conversion.
(b) Concerted feed back regulation. [10+6]
3. Define bioconversion, and list out in detail the advantages of bio conversion in
pharmaceutical industry with suitable examples. [16]
4. What is enzyme inhibition and detail the various modes of enzyme inhibition? [16]
5. Compare and contrast indirect and direct fermentations citing amino acid synthesis
as an example. [16]
6. List out biotechnological applications of enzymes (ex:- Proteases, Amylases etc.)
produced by fermentation. [16]
7. Detail on (with examples)
(a) Active transport
(b) Group transport. [8+8]
8. List out the organisms involved in production of secondary metabolites with detail
on one secondary metabolite as an example. [16]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.2
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. How can Metabolic pathways be genetically controlled. Explain with 2 examples.
[16]
2. Write in detail on:
(a) Passive diffusion
(b) Isozymes. [8+8]
3. Explain gene regulation by Jacob-Monad model citing ‘Lac’ operon as example.
[16]
4. Detail how gene dosage is evaluated and how does gene dosage effect fermentation
process. [16]
5. Describe in detail the various modes of diffusion. [16]
6. Explain the Fermentation parameters involved in production of wine from yeast.
[16]
7. Distinguish and differentiate concerted feed back regulation and cumulative feed
back regulation with examples. [16]
8. How can Mutation effect the Enzyme production of an organism. [16]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.3
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. List out and describe the parameters involved in scale-up of Fermentation process
from pilot scale. [16]
2. Describe
(a) Gene dosage
(b) Amino acid regulation of RNA synthesis. [10+6]
3. Explain mixed OG sequential bioconversions with suitable examples. [16]
4. Describe catabolite repression with tryptophan operon as example. [16]
5. Define mutation and various modes of generating mutations in improving biotech-
nology for an organism. [16]
6. Describe induction and repression phenomena with E.coli as example. [16]
7. How can permeability of an organism be altered in focus with primary metabolites.
[16]
8. Write a detailed note on:
(a) Glucose effect
(b) Active transport. [8+8]
? ? ? ? ?
1 of 1
Code No: RR412311 Set No.4
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Describe in detail the secondary screening involved in strain selection with an ex-
ample. [16]
2. Write a detailed note on:
(a) CAMP
(b) Feed back repression. [10+6]
3. Define bioconversion and describe the factors involved in bioconversion with an
example. [16]
4. What is antibiotic fermentation and explain it’s methodology in pencillin produc-
tion. [16]
5. List out and detail the factors contributing to catalytic efficiency of Enzymes. [16]
6. How do mutations effect the enzyme production. List out the aspects involved in
optimization of mutants for high yield protein production. [16]
7. Describe in detail the conversion of insoluble substances by mixed or sequential
bioconversions. [16]
8. Write a detailed note on:
(a) Fed-batch fermentation
(b) Continuous fermentation. [8+8]
? ? ? ? ?