MOLECULAR BIOLOGY

Code No: R05222303 Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Represent a melting curve of a double helix DNA and explain it in detail? [16]
2. Write a note on the famous Messelson & Stahl experiment on proving semi conser-
vative mode of DNA replication. [16]
3. What is the mechanism & importance of “proof reading” function of DNA poly-
merase in DNA replication? [16]
4. With respect to tRNA structure, explain:
(a) Stems& loops
(b) Anticodon arm. [2 × 8 = 16]
5. Which are the different types of rRNA associated with eukaryotic ribosome? How
are these synthesized? [16]
6. How are Wobble hypothesis and the genetic code correlated with each other? [16]
7. How is the process of eukaryotic process of translation different from prokaryotic
process. [16]
8. With context to mutations, what is meant by: [2 × 8 = 16]
(a) Base substitutions
(b) Insertions and deletions.
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Code No: R05222303 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Which form of DNA exists as the natural form present in living cells? Explain in
detail. [16]
2. What is meant by “rolling circle” form of DNA replication? Give an example. [16]
3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of
telomase in it. [16]
4. How are eukaryotic and prokaryotic RNA polymerases different from each other?
[16]
5. What is the relevance of intron and exon sequences with respect to the final mRNA
formation in eukaryotic posttranscriptional processing? [16]
6. Explain how the genetic information present in the mRNA is decoded into amino
acid sequence during the process of translation? [16]
7. What are post transnational modifications? Give some examples. [16]
8. Briefly explain the different DNA repair mechanisms existing in a cell. [16]
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Code No: R05222303 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. what is the primary structure of DNA? Explain in detail. [16]
2. Write a note on the famous Messelson & Stahl experiment on proving semi conser-
vative mode of DNA replication. [16]
3. Discuss about the various enzymes involved in carrying out the process of the DNA
replication. [16]
4. Discuss about the different types of RNA and the RNA Polymerases, which are
involved in synthesizing them. [16]
5. Write a note on how tRNA is produced by processing larger pre-tRNA transcript.
[16]
6. How are Wobble hypothesis and the genetic code correlated with each other? [16]
7. Explain how conjugation is a process through which genetic recombination is pos-
sible in bacteria? [16]
8. With reference to acting as mutagens, explain: [2 × 8 = 16]
(a) Uv irradiation
(b) Ionizing radiation.
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Code No: R05222303 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the main functions of DNA in a living cell? [16]
2. How does DNA replication occur in Mitochondria DNA? [16]
3. What are Telomers in DNA? How do they replicate. [16]
4. Write notes on:
(a) Prokaryotic RNA polymarase
(b) Eukaryotic RNA polymarase. [2 × 8 = 16]
5. Explain the sequence by which introns are removed from a eukaryotic pre-mRNA.
[16]
6. Elaborate the statement “Genetic code is a triplet, redundant, nonoverlapping &
comma free code”. [16]
7. Write short notes on: [2 × 8 = 16]
(a) tRNA as an adaptor molecule
(b) Difference between prokaryotic and eukaryotic ribosomes.
8. With reference to acting as mutagens, explain: [2 × 8 = 16]
(a) Uv irradiation
(b) Ionizing radiation.
⋆ ⋆ ⋆ ⋆ ⋆
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POSTED BY VAGDEVI AT 9:44 AM 0 COMMENTS
LABELS: MOLECULAR BIOLOGY
REACTIONS:
MOLECULAR BIOLOGY-SUPPLY 2K8
Code No: RR222305 Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What is hybridization? What is the chemical basis of molecular hybridization?
[16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. Write the differences between the eukaryotic and prokaryotic RNA polymerases and
their mechnism? [16]
4. Describe the following terms: Transcription, translation, promoter. [16]
5. What are mutagens? Classify the radiation, chemical mutagens that are affecting
the organisms. [16]
6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. What differenciates specialized, generalized and Abortive transduction processes?
[16]
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Code No: RR222305 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. In what component parts do DNA and RNA differs? [16]
2. What are the differences between continuous and discontinuous DNA replication?
Why does it exist? [16]
3. How does this RNA act as an RNA polymerase? What are the reactions? How
does it differ from a protein RNA polymerase? How is it similar? [16]
4. Discuss the role of the promoter and ribosome sites in regulating gene expression.
[16]
5. Write short notes on
(a) Thymine dimers
(b) Error prone Repair [16]
6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]
7. How do you map the chromosome in Hfr bacteria? [16]
8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirectional?
[16]
3. What processing events occur with prokaryotic mRNA? [16]
4. Describe the following terms: Transcription, translation, promoter. [16]
5. Write an essay that describes mutations. [16]
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]
7. Describe the conditions under which genetic recombination may occur in
bacteriophage? [16]
8. Write a note on Virus - mediated bacterial DNA transfer. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]
2. compare conservative, semiconservative and dispersive modes of DNA replication.
[16]
3. What are the general principles underlying all splicing mechanisms? [16]
4. Why would isoleucine be less likely to occur in a beta turn than in a beta strand?
[16]
5. Contrast and compare the mutagenic effects of deaminating agents, alkylating
agents and base analogous. [16]
6. Write a note on molecular structure of eukaryotic gene. [16]
7. Describe the mechanism of transformation process. [16]
8. Outline the steps involved in the replication of a lytic bacteriophage. [16]
⋆ ⋆ ⋆ ⋆ ⋆
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POSTED BY VAGDEVI AT 9:43 AM 0 COMMENTS
LABELS: MOLECULAR BIOLOGY
REACTIONS:
MOLECULAR BIOLOGY-SUPPLY 2K7
Code No: R05222303 Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What does “right handed helix” & “left handed helix” mean? Explain taking
suitable examples. [16]
2. How does rolling circle replication produce multimers of a replicon? [16]
3. How is the lagging strand synthesis different from the leading strand synthesis in
DNA replication? [16]
4. Justify the statement “there are different types of rRNA found in ribosome” [16]
5. Using suitable examples explain how they are acting as inhibitors of transcription.
[16]
6. What is meant by “Wobble hypothesis”? Explain its connection to the genetic
code. [16]
7. How is the initiation of translation process occurring in prokaryotes and eukaryotes?
Elaborate briefly. [16]
8. Write short notes on [2 × 8 = 16]
(a) Thiamine dimmers and photo reactivation
(b) Excision type of DNA repair
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Code No: R05222303 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What does “right handed helix” & “left handed helix” mean? Explain taking
suitable examples. [16]
2. Using neat diagrams explain a circular chromosome undergoing bi-directional semi
conservative replication. [16]
3. Explain the process of DNA replication in detail. [16]
4. Discuss in detail the structure of the organelles functioning as sites of protein
synthesis. [16]
5. Using suitable examples explain how they are acting as inhibitors of transcription.
[16]
6. In the genetic code, how can one justify the presence of 64 codons coding for only
20 amino acids. [16]
7. Once a protein is synthesized, can it be modified? Justify your answer giving
suitable examples. [16]
8. With the help of a neat diagram explain how the process of site directed mutagenesis
is done. [16]
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Code No: R05222303 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write a note on the different forms of the double helix DNA and elaborate on “B”
form. [16]
2. How does the bacteriophage ×174 replicate through rolling circle mechanism?
[16]
3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of
telomase in it. [16]
4. Write about the different types of ribonucleic acids. [16]
5. Where does the synthesis & processing of pre-rRNA occur? Explain the process.
[16]
6. Write briefly about the steps of initiation, elongation and termination in the process
of prokaryotic translation. [16]
7. What are post transnational modifications? Give some examples. [16]
8. How is reverse genetics approach used to study a gene function? [16]
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Code No: R05222303 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Explain how GC base pair content of a ds DNA affects the melting temperature
Tm. [16]
2. With respect to DNA replication, explain
(a) Origin of replication
(b) Replication fork
(c) Bidirectional replication
(d) Semi-conservative replication. [4 × 4 = 16]
3. How does DNA replication proceed in the 5’ − > 3’ polarity parental DNA strand?
[16]
4. Give a step-by-step description of the process of Transcription. [16]
5. Describe briefly about the posttranscriptional processing occurring in eukaryotic
mRNA. [16]
6. In the genetic code, how can one justify the presence of 64 codons coding for only
20 amino acids. [16]
7. With context to gene transfer mechanisms, explain [2 × 8 = 16]
(a) Transduction
(b) Conjugation
8. Explain how base analogs and alkylating agents act as chemical mutagens in in-
duced mutations. [16]
⋆ ⋆ ⋆ ⋆ ⋆
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POSTED BY VAGDEVI AT 9:42 AM 0 COMMENTS
LABELS: MOLECULAR BIOLOGY
REACTIONS:
MOLECULAR BIOLOGY-SUPPLY 2K6
Code No: RR222305 Set No. 1
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What is the relationship between nucleosomes, 30 nm fibers and the scaffold struc-
ture with respect to the organization of DNA in the nucleus? [16]
2. Why is the 3’OH group on the ribose ring so important for DNA and RNA synthe-
sis? [16]
3. Eukaryotic mRNA is usually monocistronic. What does this mean? Why is this
the case for eukaryotes, whereas polycistronic mRNA is often found in prokaryotes?
[16]
4. Describe the following terms ribosome binding site, RNA polymerase, tRNA syn-
thetases. [16]
5. Write short notes on
(a) Thymine dimers
(b) Error prone Repair [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 2
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the roles of RNA primers and Okazaki fragments during DNA replication?
[16]
2. Describe Meselson-Stahl experiment. [16]
3. Write the following
(a) RNA Pol “basal” factors
(b) .RNA Pol “Transcription factors”? [16]
4. Write briefly on the different types of protein that exists. [16]
5. What is significantly different about the type of DNA “damage” repaired by mis-
match repair compared to other types of DNA damage? [16]
6. Write a note on microbial testing that are widely used in industrial research.
[16]
7. What is the role of Rec protein in bacterial recombination? [16]
8. Describe lysis and lysogeny. [16]
⋆ ⋆ ⋆ ⋆ ⋆
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Code No: RR222305 Set No. 3
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. Write short notes on
(a) Rho dependent termination.
(b) Rho independent termination. [16]
4. What will determine whether regions of alpha-helical structure lie at the surface or
in the interior of a water-soluble globular protein? [16]
5. What is meant by Reverse Genetics? How is useful? [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. With respect to F+ and F− bacterial mating, answer the followings
(a) How was it established that physical contact was necessary?
(b) How was it established that chromosome transfer was unidirectional?
[16]
8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 4
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Summarize and compare the properties of DNA polymerase- I, II and III. [16]
2. In Meselson-Stahl experiment, which of the three modes of replication could be
ruled out after one round of replication? After two round? [16]
3. What processing events occur with prokaryotic mRNA? [16]
4. Which feature of the Genetic code is responsible for redundancy of Geretic Code.
[16]
5. What is significantly different about the type of DNA “damage” repaired by mis-
match repair compared to other types of DNA damage? [16]
6. Write a note on split genes. [16]
7. What is the role of Rec protein in bacterial recombination? [16]
8. Describe what are the three different physical forms of phage λ chromosome?
[16]
⋆ ⋆ ⋆ ⋆ ⋆
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POSTED BY VAGDEVI AT 9:41 AM 0 COMMENTS
LABELS: MOLECULAR BIOLOGY
REACTIONS:
MOLECULAR BIOLOGY-SUPPLY 2K5
Code No: RR222305 Set No. 1
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe the various characteristics of the Watson-Crick DNA model. [16]
2. What are the requirements for the in vitro synthesis of DNA under the direction
of DNA polymerase-I? [16]
3. What is a “TATA box” and what is its function? Do all promoters have TATA
boxes? Why or why not? What provides the function of the TATA box when it
isn’t present? [16]
4. Describe the following terms: Transcription, translation, promoter. [16]
5. Describe different types of mutations; and explain the importance of mutations for
genetic research. [16]
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Write a note on Virus - mediated bacterial DNA transfer. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 2
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the roles of RNA primers and Okazaki fragments during DNA replication?
[16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. Where are each of the RNA polymerase types found in the eukaryotic cell? [16]
4. What is footprinting? How did it help define promoter sequences? [16]
5. What are mutagens? Classify the radiation, chemical mutagens that are affecting
the organisms. [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Why are IS elements sometimes referred to as selfish DNA? [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 3
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Summarize and compare the properties of DNA polymerase- I, II and III. [16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. What processing events occur with prokaryotic mRNA? [16]
4. Argue why the genetic code must be read 3 bases at a time rather than 2 bases.
[16]
5. Write short notes on
(a) Thymine dimers
(b) Error prone Repair [16]
6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. What are the properties of viruses? How do viruses differ from all type of cells?
Why are viruses called obligate intracellular parasites? [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222305 Set No. 4
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe the various characteristics of the Watson-Crick DNA model. [16]
2. What are two differences between DNA and RNA? What bases pair with each
other? [16]
3. Where are each of the RNA polymerase types found in the eukaryotic cell? [16]
4. Write a note on post-transnational modification. [16]
5. Describe different types of mutations; and explain the importance of mutations for
genetic research. [16]
6. Which are the functional portions of a functional gene? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Describe the mechanism of transduction process. [16]
⋆ ⋆ ⋆ ⋆ ⋆
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LABELS: MOLECULAR BIOLOGY
REACTIONS:
MOLECULAR BIOLOGY-REG 2K5
Code No: RR222305 Set No.1
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write the detail components of DNA molecule that are organized inside cell.
2. compare conservative, semiconservative and dispersive modes of DNA replication.
3. How does the structure of a eukaryotic CoreRNAPol compare with
the E. coli CoreRNApol?
4. Discuss the roles of the following in protein expression: rRNA, tRNA, mRNA.
5. Write an essay that describes mutations.
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. Distinguish among the three modes of recombination in bacteria.
8. Describe what are the three different physical forms of phage chromosome?
? ? ? ? ?
1 of 1
Code No: RR222305 Set No.2
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. In what component parts do DNA and RNA differs?
2. Describe Meselson-Stahl experiment.
3. Write the following
(a) RNA Pol “basal” factors
(b) .RNA Pol “Transcription factors”?
4. Why would isoleucine be less likely to occur in a beta turn than in a beta strand?
5. Write an essay that describes mutations.
6. Write a note on molecular structure of eukaryotic gene.
7. Why are the recombinants produced from an HfrXF− cross never F+?
8. Describe the methods of transduction.
? ? ? ? ?
1 of 1
Code No: RR222305 Set No.3
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. If the tetranucleotide hypothesis were correct regarding the simplicity of DNA
structure, under what circumstances could DNA be the genetic material.
2. What are two differences between DNA and RNA? What bases pair with each
other?
3. Where are each of the RNA polymerase types found in the eukaryotic cell?
4. Name and describe the 4 levels of protein structure?
5. Most mutations are thought to be deleterious. Why then, is it reasonable to state
that mutations are essential for evolutionary process?
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. What do you mean by plasmid? Describe their role in recombination.
8. Why are IS elements sometimes referred to as selfish DNA?
? ? ? ? ?
1 of 1
Code No: RR222305 Set No.4
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. What are the roles of RNA primers and Okazaki fragments during DNA replica-
tion?
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional?
3. Write short notes on
(a) Rho dependent termination.
(b) Rho independent termination.
4. What will determine whether regions of alpha-helical structure lie at the surface or
in the interior of a water-soluble globular protein?
5. Describe different types of mutations; and explain the importance of mutations for
genetic research.
6. Which are the functional portions of a functional gene?
7. Describe the mechanism of transformation process.
8. What are Retroposons?
? ? ? ? ?
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Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. In what component parts do DNA and RNA differ?
2. What did the Watson-Crick model suggest about the replication of the DNA?
3. What is the process of removal of introns from pre-mRNA called? Where does it
occur? Where do other eukayrotic mRNA modifications take place?
4. Describe the functions of signal sequences and stop transfer sequences in the
export of proteins from the cytoplasm.
5. What is the role of the "DNA template" in excision repair? What is this
"template"?
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic?
7. Write a note on genetic recombination in Bacteria.
8. Describe the Shapiro model of transposition. What are the roles of transposase,
DNA polymeraseI, Ligase and resolvase?
*$*$*$*
Set No.
1
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. Sketch the shape of B-DNA and Z-DNA and write their properties.
2. Compare the conservative, semi-conservative, and dispersive modes of DNA
replication.
3. What is splicing and alternative splicing? Give examples of different types of
alternative splicing.
4. Explain the difference between unregulated protein production, transcriptional
regulation, translational regulation, and post-translational regulation.
5. Which of the proteins is an ATPase? What is an ATPase? Why is an ATPase
needed in excision repair?
6. Write a note on microbial testing that are widely used in industrial research.
7. Describe the conjugation process in F+ and F- bacteria.
8. How do transpons induce deletion and inversion?
*$*$*$*
Set No.
2
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. Describe the various characteristics of the Watson-Crick DNA model.
2. Describe Meselson-Stahl experiment.
3. What is hetergeneous nuclear RNA? How is it related to eukaryotic mRNA?
4. Describe the following terms: Transcription, translation, promoter.
5. What is the primary difference between transcription-coupled repair in
prokaryotes and in eukaryotes?
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. How do you map the chromosome in Hfr bacteria?
8. Describe how a transposon controls the expression of the flagellar phase in
salmonella.
*$*$*$*
Set No.
3
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. What the physical state of DNA following denaturation?
2. In Meselson-Stahl experiment, which of the three modes of replication could be
ruled out
a) After one round of replication.
b) After two round.
3. What are the 5 general mechanisms used in RNA splicing? Explain each of them.
4. Describe the following terms ribosome binding site, RNA polymerase, tRNA
synthetases.
5. What is significantly different about the type of DNA "damage" repaired by
mismatch repair compared to other types of DNA damage?
6. Write a note of Benzer's fine structure analysis of the rII locus of T4
bacteriophage.
7. Describe the recombination process in F+ and F- mating.
8. Describe what are the three different physical forms of phage l chromosome.
*$*$*$*